Continuous-flow and Label-free Ferrohydrodynamic Sorting of Mammalian Cells in Biocompatible Ferrofluids
نویسندگان
چکیده
This paper reports a new continuous-flow and label-free sorting scheme based on biocompatible ferrofluid hydrodynamics (ferrohydrodynamics) for the separation of mammalian cells (HeLa cells and mouse whole blood). Ferrofluids are stable magnetic nanoparticles suspensions used as sorting media in microfluidics for both particles and cells. Here we develop a biocompatible ferrofluid that can sustain the viability of mammalian cells for up to several hours with excellent colloidal stability and tunable concentration to allow for cell observation without fluorescent labels. We apply this ferrofluid in continuous-flow sorting of HeLa cells and blood cells with high throughput and high separation efficiency.
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